2JGB
Structure of human eIF4E homologous protein 4EHP with m7GTP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-26 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 43.499, 49.299, 103.767 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 51.920 - 1.700 |
| R因子 | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ipb |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.494 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 1.790 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.060 | 0.370 |
| 独立反射数 | 111967 | |
| <I/σ(I)> | 17.7 | 3.4 |
| 完全性 [%] | 99.6 | 99.2 |
| 冗長性 | 4.5 | 4.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 5.5 | 20% PEG-3000, 0.1M SODIUM CITRATE PH 5.5 WITH 1MM M7GTP IN THE DROP |
