2J70
Structural and functional characterisation of partner-switching regulating the environmental stress response in B. subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-09 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.137, 45.422, 76.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.460 - 1.950 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w53 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.773 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.170 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 0.220 |
Number of reflections | 5626 | |
<I/σ(I)> | 6.4 | 2.7 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 10 % PEG 20000, 100 MM MES PH 6.5 |