2IZZ
Crystal structure of human pyrroline-5-carboxylate reductase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 110.453, 177.944, 87.916 |
| 格子定数 [度] | 90.00, 106.70, 90.00 |
精密化法
| 残基 | 40.000 - 1.950 |
| R因子 | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ag8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 2.060 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.060 | 0.380 |
| 独立反射数 | 117744 | |
| <I/σ(I)> | 11.9 | 2.3 |
| 完全性 [%] | 99.9 | 99.9 |
| 冗長性 | 3.1 | 2.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7.5 | 20% PEG3350, 10% ETHYLENE GLYCOL 0.2M NA2SO4, 0.1M BIS-TRIS PROPANE PH7.5, pH 7.50 |
