2IWO
12th PDZ domain of Multiple PDZ Domain Protein MPDZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-16 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.483, 76.403, 32.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.300 - 1.700 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fne |
RMSD bond length | 0.019 |
RMSD bond angle | 1.626 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.210 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.100 | 0.430 |
Number of reflections | 21145 | |
<I/σ(I)> | 11 | 3.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 2M (NH4)2SO4, pH 7.50 |