2IQJ
Crystal structure of the GAP domain of SMAP1L (LOC64744) stromal membrane-associated protein 1-like
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-20 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.54180 |
Spacegroup name | P 1 |
Unit cell lengths | 34.812, 38.513, 47.386 |
Unit cell angles | 90.10, 108.89, 105.13 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2crr |
RMSD bond length | 0.017 |
RMSD bond angle | 1.358 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
Rmerge | 0.052 | 0.029 | 0.381 |
Number of reflections | 17983 | 1890 | 1731 |
<I/σ(I)> | 14.3 | ||
Completeness [%] | 94.1 | ||
Redundancy | 3.9 | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG-3350, 0.2M Ammonium acetate, 0.1M Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K |