2I1J

Moesin from Spodoptera frugiperda at 2.1 angstroms resolution

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	112
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	H 3 2
格子定数 [Å]	123.739, 123.739, 283.183
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.100
R因子	0.176
Rwork	0.179
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	42.720
分解能 [Å] (高)	2.000
独立反射数	43611

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8	277	100 mM HEPES pH 8.0, 15% PEG 8000, 50 mM phosphoserine, 800 mM NaCl, 1 M urea, VAPOR DIFFUSION, HANGING DROP, temperature 277K