2HTV

N4 neuraminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-05
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	P 4 3 2
Unit cell lengths	193.790, 193.790, 193.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.222
Rwork	0.222
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	29345
<I/σ(I)>	8
Completeness [%]	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K