2HS3
T. maritima PurL complexed with FGAR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.672, 57.355, 75.451 |
Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
Resolution | 44.060 - 2.300 |
R-factor | 0.1931 |
Rwork | 0.193 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.060 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 18741 | |
Completeness [%] | 76.8 | 75.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 17% PEG 4000, 4% isopropanol, 0.1M HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |