2H9U
Crystal structure of the archaea specific DNA binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.130, 55.040, 33.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.720 - 2.000 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hox |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data scaling software | SCALA |
Phasing software | MOLREP (MR) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.054 | 0.334 |
Number of reflections | 10332 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG4000, Ammonium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |