2H4O
X-ray Crystal Structure of Protein yonK from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR415
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97913, 0.97941, 0.96780 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.016, 72.201, 48.935 |
| Unit cell angles | 90.00, 113.78, 90.00 |
Refinement procedure
| Resolution | 28.340 - 2.800 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.30100 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.048 | 0.116 |
| Number of reflections | 15092 | |
| <I/σ(I)> | 17.7 | 16.1 |
| Completeness [%] | 1.0 | 0.99 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 1000, 100mM TAPS pH 9.0, 120mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
