2H0H
Crystal Structure of DsbG K113E mutant
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 117.110, 56.980, 85.003 |
| 格子定数 [度] | 90.00, 94.84, 90.00 |
精密化法
| 残基 | 20.000 - 1.800 |
| Rwork | 0.197 |
| R-free | 0.22900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1v57 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.200 |
| Data reduction software | MX (SYSTEM) |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 1.860 |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmerge_l_obs | 0.063 | 0.320 |
| 独立反射数 | 52294 | |
| <I/σ(I)> | 20 | 3 |
| 完全性 [%] | 99.6 | 97.4 |
| 冗長性 | 4.1 | 3.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 277 | 17% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
