2GMH
Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 1.7389, 1.7426, 1.6000 |
| Spacegroup name | P 4 21 2 |
| 格子定数 [Å] | 154.322, 154.322, 128.536 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 29.670 - 2.500 |
| R因子 | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 3.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 29.670 | 2.540 |
| 分解能 [Å] (高) | 2.500 | 2.500 |
| 独立反射数 | 53508 | |
| <I/σ(I)> | 35.3 | 7.6 |
| 完全性 [%] | 98.7 | 97.4 |
| 冗長性 | 11.9 | 4.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEGMME 2000, Sodium Chloride, Ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
