2GMH
Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0, 1.7389, 1.7426, 1.6000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 154.322, 154.322, 128.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.670 - 2.500 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 3.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.670 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 53508 | |
<I/σ(I)> | 35.3 | 7.6 |
Completeness [%] | 98.7 | 97.4 |
Redundancy | 11.9 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEGMME 2000, Sodium Chloride, Ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |