2GLK
High-resolution study of D-Xylose isomerase, 0.94A resolution.
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 92.712, 97.935, 101.867 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 35.360 - 0.940 |
| R因子 | 0.1157 |
| Rwork | 0.115 |
| R-free | 0.12792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xib |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.021 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.917 |
| Data reduction software | X-GEN |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 35.360 | 0.965 |
| 分解能 [Å] (高) | 0.940 | 0.944 |
| 独立反射数 | 252079 | |
| 完全性 [%] | 85.6 | 12 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 8 | 293 | 50mM tris-HCl,25%AMSO4,2mM Mn2+ and 2mM Co2+ , XI @ 25mg/ml., pH 8, VAPOR DIFFUSION, temperature 293K |
