2GJS
The crystal structure of human RRAD in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.427, 58.560, 53.603 |
Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
Resolution | 39.400 - 1.900 |
R-factor | 0.19726 |
Rwork | 0.195 |
R-free | 0.23538 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | swissmodel (first approach mode) |
RMSD bond length | 0.015 |
RMSD bond angle | 1.605 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 24816 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.15M sodium malate, 20% (w/v) PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |