2FSX
Crystal structure of Rv0390 from M. tuberculosis
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 50.814, 50.814, 119.184 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 30.400 - 1.800 |
| R因子 | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.21767 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.613 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.860 |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmerge_l_obs | 0.115 | 0.450 |
| 独立反射数 | 15238 | |
| <I/σ(I)> | 5 | 3.5 |
| 完全性 [%] | 99.9 | 98.9 |
| 冗長性 | 8 | 8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | Sodium Citrate 1.0176M, MES 0.1M, Ammonium Sulfate 0.31752M , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
