2FQE
Crystal Structures of E. coli Laccase CueO under different copper binding situations
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
格子定数 [Å] | 69.587, 73.205, 184.324 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 92.060 - 1.920 |
Rwork | 0.165 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 1.129 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 100.000 | 100.000 | 1.940 |
分解能 [Å] (高) | 1.900 | 4.690 | 1.900 |
Rmerge_l_obs | 0.109 | 0.066 | 0.401 |
独立反射数 | 35855 | 2550 | 2341 |
<I/σ(I)> | 6.7 | ||
完全性 [%] | 99.8 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 10% polyethylene glycol 4000, 15% Isopropanol, 100mM tri-Sodium Citrate dihydrate pH5.6, 0.5mM CuCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |