2FP4
Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GTP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 132.610, 132.610, 72.530 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 116.038 - 2.080 |
| R因子 | 0.214 |
| Rwork | 0.195 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 116.038 | 72.990 | 2.210 |
| 分解能 [Å] (高) | 2.080 | 6.640 | 2.100 |
| Rmerge_l_obs | 0.055 | 0.019 | 0.362 |
| Total number of observations | 8067 | 22254 | |
| 独立反射数 | 43486 | 1442 | 6210 |
| <I/σ(I)> | 11.9 | 28.6 | 2 |
| 完全性 [%] | 99.3 | ||
| 冗長性 | 5.5 | 5.6 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 5 mM GTP, 10 mM MgCl2, 20% w/v polylethylene glycol 3350, 150 mM KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
