2FH7
Crystal structure of the phosphatase domains of human PTP SIGMA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 |
| 格子定数 [Å] | 94.333, 94.333, 123.036 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 24.670 - 2.000 |
| R因子 | 0.19199 |
| Rwork | 0.190 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lar |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 25.760 | 1.910 |
| 分解能 [Å] (高) | 1.810 | 1.810 |
| Rmerge_l_obs | 0.093 | 0.011 |
| 独立反射数 | 55608 | |
| <I/σ(I)> | 6 | 0.6 |
| 完全性 [%] | 99.0 | 93.3 |
| 冗長性 | 9.3 | 6.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | Protein(10mg/ml) was crystallized in (15% PEG3350,100mM Succinic Acid)., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
