2F5Y
Crystal Structure of the PDZ Domain from Human RGS-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.110, 44.140, 49.857 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.880 - 2.390 |
R-factor | 0.21698 |
Rwork | 0.215 |
R-free | 0.26429 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1I92.pdb 1QAU.pdb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.484 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.860 | 2.490 |
High resolution limit [Å] | 2.390 | 2.400 |
Number of reflections | 8381 | |
Completeness [%] | 99.7 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M ammonium sulphate, 0.1M MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |