2F58
IGG1 FAB FRAGMENT (58.2) COMPLEX WITH 12-RESIDUE CYCLIC PEPTIDE (INCLUDING RESIDUES 315-324 OF HIV-1 GP120) (MN ISOLATE)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-05-15 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 96.080, 114.720, 49.530 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 24.000 - 2.800 |
| R因子 | 0.185 |
| Rwork | 0.185 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAB 58.2 PORTION OF FAB 58.2/SER-LOOP PEPTIDE COMPLEX |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.900 |
| Data reduction software | UCSD-system |
| Data scaling software | UCSD-system |
| Phasing software | MERLOT |
| Refinement software | X-PLOR (3.851) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 24.000 | 3.020 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 12923 | |
| <I/σ(I)> | 7.5 | 1 |
| 完全性 [%] | 91.9 | 82.8 |
| 冗長性 | 3.4 | 1.99 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 5 | 16% PEG4000, 0.2M IMIDAZOLE MALATE, PH 5.0 |
