2EWF
Crystal structure of the site-specific DNA nickase N.BspD6I
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9198, 0.9200, 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.553, 92.562, 76.349 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.840 |
| R-factor | 0.2 |
| Rwork | 0.183 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.080 | |
| Number of reflections | 67523 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.04 M KH2PO4, 16% (w/v) PEG 8000, 20% (v/v) glycerol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
