2ERC
CRYSTAL STRUCTURE OF ERMC' A RRNA-METHYL TRANSFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1996-10 |
| Detector | TATE ET AL. (1995) J. APPL. CRYST. 28, 196-205. |
| Spacegroup name | P 6 |
| 格子定数 [Å] | 146.900, 146.900, 57.900 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 8.000 - 3.030 |
| R因子 | 0.232 |
| Rwork | 0.232 |
| R-free | 0.31300 |
| Structure solution method | MAD PHASING IN A DIFFERENT EXPERIMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 21.850 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 3.500 | |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| Rmerge_l_obs | 0.068 | 0.027 |
| Total number of observations | 138382 * | |
| 独立反射数 | 14568 | |
| <I/σ(I)> | 10 | 2 |
| 完全性 [%] | 94.0 | 84.3 |
| 冗長性 | 9.5 | 6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 4.0 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | SAH | 40-fold molar excess | |
| 7 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 8 | 1 | reservoir | citrate | 0.1 (M) |
