2ECF

Crystal Structure of Dipeptidyl Aminopeptidase IV from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-03-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	105.877, 105.877, 161.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.185
R-free	0.24000
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.310
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	23396
<I/σ(I)>	8.3	2.2
Completeness [%]	100.0	99.8
Redundancy	7.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	12% PEG 6000, 0.2M magnesium chloride, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8	293	12% PEG 6000, 0.2M magnesium chloride, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8	293	12% PEG 6000, 0.2M magnesium chloride, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, temperature 293K