2EA3
Crystal Structure Of Cellulomonas Bogoriensis Chymotrypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.190, 52.490, 76.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.100 - 1.780 |
| R-factor | 0.16714 |
| Rwork | 0.166 |
| R-free | 0.19517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2sga |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.160 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.021 |
| Number of reflections | 13319 |
| <I/σ(I)> | 34 |
| Completeness [%] | 93.1 |
| Redundancy | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 8000, 0.2M ammonium sulphate, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
