2DT5
Crystal Structure of TTHA1657 (AT-rich DNA-binding protein) from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 135.595, 135.595, 68.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.630 - 2.160 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.23800 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.240 |
High resolution limit [Å] | 2.160 | 2.160 |
Number of reflections | 34573 | |
<I/σ(I)> | 47.9 | 8.8 |
Completeness [%] | 99.6 | 99 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | LITHIUM SULFATE, HEPES-NAOH, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |