2DLF
HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 6.75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 112 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.290, 66.930, 53.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.550 |
| R-factor | 0.179 * |
| Rwork | 0.184 |
| R-free | 0.22800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.520 |
| High resolution limit [Å] | 1.550 * | 1.550 * |
| Rmerge | 0.052 * | 0.318 |
| Total number of observations | 331744 * | |
| Number of reflections | 29973 * | |
| <I/σ(I)> | 42.6 | 3.5 |
| Completeness [%] | 97.7 * | 96.3 * |
| Redundancy | 11.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 293 * | CRYSTALLIZATION CONDITIONS: TEMPERATURE AT 20C. PRECIPITANT SOLUTION CONTAINED 1 AMMONIUM SULFATE AND 0.025 M SODIUM CACODYLATE (PH 6.75). 5 MG/ML OF FV FRAGMENT., VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.8 (M) | |
| 3 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 25 (mM) |
