2D2A
Crystal Structure of Escherichia coli SufA Involved in Biosynthesis of Iron-sulfur Clusters
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-29 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.162, 88.500, 122.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.600 - 2.700 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s98 |
RMSD bond length | 0.047 |
RMSD bond angle | 3.400 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.085 | 0.349 |
Number of reflections | 8136 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | PEG2000MME, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |