2CMG
Crystal Structure of Spermidine Synthase from Helicobacter Pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17B2 |
Synchrotron site | NSRRC |
Beamline | BL17B2 |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.147, 58.141, 74.832 |
Unit cell angles | 90.00, 90.93, 90.00 |
Refinement procedure
Resolution | 24.830 - 2.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.27300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.040 | 0.270 |
Number of reflections | 32833 | |
<I/σ(I)> | 19.7 | 3.4 |
Completeness [%] | 91.9 | 87 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.50 |