2CG4
Structure of E.coli AsnC
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 4 |
| 格子定数 [Å] | 103.117, 103.117, 52.401 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 30.000 - 2.400 |
| R因子 | 0.232 |
| Rwork | 0.229 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LOW RES ASNC FROM SELENO MET EXPERIMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.971 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.530 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.100 | 0.690 |
| 独立反射数 | 21843 | |
| <I/σ(I)> | 9.8 | 1.7 |
| 完全性 [%] | 99.2 | 99.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 8.7 | 2.2M MAGNESIUM CHLORIDE, O.1M BICINE PH 8.7, 5MM L-ASPARAGINE |
