2C56

A comparative study of uracil DNA glycosylases from human and herpes simplex virus type 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	42.040, 61.117, 43.392
Unit cell angles	90.00, 92.25, 90.00

Refinement procedure

Resolution	29.590 - 2.100
R-factor	0.151
Rwork	0.151
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1udg
RMSD bond length	0.016
RMSD bond angle	1.700
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.220
Number of reflections	12927
<I/σ(I)>	12.56	5.26
Completeness [%]	100.0	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1