2C0Z
The 1.6 A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-25 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 59.354, 59.354, 109.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.990 - 1.600 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ep0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.492 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 26449 | |
<I/σ(I)> | 25.7 | 3.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 25% SATURATED AMMONIUM SULFATE IN 100MM TRIS-HCL PH8.5, pH 8.50 |