2BUJ

Crystal structure of the human Serine-threonine Kinase 16 in complex with staurosporine

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-23
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
格子定数 [Å]	104.554, 168.901, 129.729
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	64.820 - 2.600
R因子	0.187
Rwork	0.185
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.536
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	64.800	2.700
分解能 [Å] (高)	2.600	2.600
Rmerge_l_obs	0.070	0.590
独立反射数	35446
<I/σ(I)>	13.52	2.29
完全性 [%]	99.3	94.3
冗長性	7.83	5.12

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP			200 NL SITTING DROP, 1.1 AMMONIUM SULFATE, 1% PEG3350, 0.1 M BIS-TRIS PH 5.0