2BPM

STRUCTURE OF CDK2-CYCLIN A WITH PHA-630529

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-28
Detector	ADSC CCD
Spacegroup name	P 62 2 2
Unit cell lengths	183.614, 183.614, 214.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.760 - 2.400
Rwork	0.229
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyw
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.597
Number of reflections	82891
<I/σ(I)>	16.87	3.8
Completeness [%]	99.9	98.8
Redundancy	8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7