2BPM
STRUCTURE OF CDK2-CYCLIN A WITH PHA-630529
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| 格子定数 [Å] | 183.614, 183.614, 214.129 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 29.760 - 2.400 |
| Rwork | 0.229 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyw |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNX (2002) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.490 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.073 | 0.597 |
| 独立反射数 | 82891 | |
| <I/σ(I)> | 16.87 | 3.8 |
| 完全性 [%] | 99.9 | 98.8 |
| 冗長性 | 8 | 7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7 | 20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7 |
