2B6O
Electron crystallographic structure of lens Aquaporin-0 (AQP0) (lens MIP) at 1.9A resolution, in a closed pore state
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Source details | JEM3000SFF |
| Temperature [K] | 281 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | GATAN |
| Spacegroup name | P 4 2 2 |
| 格子定数 [Å] | 65.500, 65.500, 160.000 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 5.000 - 1.900 |
| Rwork | 0.258 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SOR - aquaporin-0 (MIP) strructure determined by electron crystallography |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.900 |
| Data reduction software | GATAN |
| Data scaling software | GATAN |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.000 |
| 分解能 [Å] (高) | 1.900 | 1.900 |
| Rmerge_l_obs | 0.166 | |
| 完全性 [%] | 80.0 | 70.5 |
| 冗長性 | 5.7 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | MICRODIALYSIS | 6 | 300 | 20mM MES pH 6.0, 50mM MgCl2, 5mM DTT, MICRODIALYSIS, temperature 300K |
