2AS5
Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1070 |
Spacegroup name | P 1 21 1 |
格子定数 [Å] | 65.455, 157.447, 67.666 |
格子定数 [度] | 90.00, 118.67, 90.00 |
精密化法
残基 | 30.000 - 2.700 |
R因子 | 0.2382 |
Rwork | 0.238 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a02 chain N |
結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
結合角の平均二乗偏差(RMSD) [度] | 1.306 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 30.000 | 2.800 |
分解能 [Å] (高) | 2.700 | 2.700 |
Rmerge_l_obs | 0.085 | |
独立反射数 | 31620 | |
<I/σ(I)> | 10.6 | |
完全性 [%] | 96.0 | 86.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | Cacodylic Acid, PEG 4k, Sodium Chloride, Magnesium Chloride, Glycerol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | Cacodylic Acid | ||
10 | 1 | 2 | Magnesium Chloride | ||
2 | 1 | 1 | PEG 4k | ||
3 | 1 | 1 | Sodium Chloride | ||
4 | 1 | 1 | Magnesium Chloride | ||
5 | 1 | 1 | Glycerol | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | Cacodylic Acid | ||
8 | 1 | 2 | PEG 4k | ||
9 | 1 | 2 | Sodium Chloride |