2A4G

Hepatitis C Protease NS3-4A serine protease with Ketoamide Inhibitor SCH225724 Bound

Experimental procedure

実験手法	SINGLE WAVELENGTH
Detector technology	IMAGE PLATE
Collection date	1999-04-08
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3 2
格子定数 [Å]	224.392, 224.392, 75.334
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	8.000 - 2.500
Rwork	0.193
R-free	0.26500
Structure solution method	FOURIER SYNTHESIS
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.850
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (98.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.590
分解能 [Å] (高)	2.500	2.500
Rmerge_l_obs		0.031
独立反射数	27752
<I/σ(I)>	17.7
完全性 [%]	99.5	99.5
冗長性	3.4	3.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.5	285	MES, sodium potassium phosphate, sodium chloride, 2-mercaptoethanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K