2A26
Crystal structure of the N-terminal, dimerization domain of Siah Interacting Protein
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.826533 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 58.793, 152.609, 36.988 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 30.000 - 1.200 |
| R因子 | 0.17489 |
| Rwork | 0.174 |
| R-free | 0.19833 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.936 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.220 |
| 分解能 [Å] (高) | 1.200 | 1.200 |
| Rmerge_l_obs | 0.046 | 0.381 |
| 独立反射数 | 50772 | |
| <I/σ(I)> | 65 | 2 |
| 完全性 [%] | 96.7 | |
| 冗長性 | 8.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | ammonium sulphate, CAPS, lithium sulphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | ammonium sulphate, CAPS, lithium sulphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
