1ZAP
SECRETED ASPARTIC PROTEASE FROM C. ALBICANS
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 63.600, 98.260, 49.280 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 8.000 - 2.500 |
| R因子 | 0.145 |
| Rwork | 0.145 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 26.780 * |
| Data reduction software | DENZO ((HKL)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 8.000 |
| 分解能 [Å] (高) | 2.500 |
| Rmerge_l_obs | 0.099 |
| Total number of observations | 30743 * |
| 独立反射数 | 10193 |
| 完全性 [%] | 89.0 |
| 冗長性 | 3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | unknown * | 4.5 * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | 1 | protein | 5-10 (mg/ml) | |
| 2 | 1 | 1 | glycine | 25 (mM) | |
| 3 | 1 | 1 | 25 (mM) | ||
| 4 | 1 | 2 | imidazole/malate | 40 (mM) | |
| 5 | 1 | 2 | 1 (mM) | ||
| 6 | 1 | 2 | PEG8000 | 22-24 (%) |
