1Z14

Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute Virus of Mice

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.870
Spacegroup name	C 1 2 1
格子定数 [Å]	448.700, 416.500, 306.100
格子定数 [度]	90.00, 95.90, 90.00

精密化法

残基	20.000 - 3.250
Rwork	0.298
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	canine parvovirus model
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.583
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS (1.0)

Quality characteristics

	Overall
分解能 [Å] (低)	20.000
分解能 [Å] (高)	3.250
Rmerge_l_obs	0.162
独立反射数	517599
完全性 [%]	64.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5		10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7.5		10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP