1YXU
Crystal Structure of Kinase Pim1 in Complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-21 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 65 |
Unit cell lengths | 195.745, 195.745, 80.564 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.550 - 2.240 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.515 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.25) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.550 | 2.360 |
High resolution limit [Å] | 2.000 | 2.240 |
Number of reflections | 84733 | |
Completeness [%] | 97.3 | 96.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Na Acetate, Imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |