1YRL
Escherichia coli ketol-acid reductoisomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 226.882, 226.882, 118.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.480 - 2.600 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.220 |
| Data reduction software | CrystalClear (v.1.3.5) |
| Data scaling software | CrystalClear (V. 1.35 (MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.480 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.300 |
| Number of reflections | 106858 | |
| <I/σ(I)> | 20.9 | 5.1 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 8.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | ammonium sulfate, sodium bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
