1YRL
Escherichia coli ketol-acid reductoisomerase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| 格子定数 [Å] | 226.882, 226.882, 118.853 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 32.480 - 2.600 |
| R因子 | 0.227 |
| Rwork | 0.223 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmg |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.220 |
| Data reduction software | CrystalClear (v.1.3.5) |
| Data scaling software | CrystalClear (V. 1.35 (MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 32.480 | 2.690 |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| Rmerge_l_obs | 0.074 | 0.300 |
| 独立反射数 | 106858 | |
| <I/σ(I)> | 20.9 | 5.1 |
| 完全性 [%] | 99.8 | 98.5 |
| 冗長性 | 8.6 | 5.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | ammonium sulfate, sodium bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
