1YNR
Crystal structure of the cytochrome c-552 from Hydrogenobacter thermophilus at 2.0 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 56.712, 56.712, 220.179 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.17287 |
Rwork | 0.171 |
R-free | 0.21777 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 451c |
RMSD bond length | 0.015 |
RMSD bond angle | 1.566 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.069 | 0.292 |
Number of reflections | 25401 | |
Completeness [%] | 98.1 | 88.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | MPD, ammonium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |