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1YF2

Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.2
Synchrotron siteALS
Beamline8.2.2
Temperature [K]100
Detector technologyCCD
Collection date2004-07-12
DetectorADSC QUANTUM 4
Wavelength(s)1.000
Spacegroup nameP 1 21 1
Unit cell lengths71.972, 94.222, 103.520
Unit cell angles90.00, 95.01, 90.00
Refinement procedure
Resolution48.520 - 2.400
R-factor0.236
Rwork0.236
R-free0.27900
Structure solution methodSAD
RMSD bond length0.012
RMSD bond angle1.200
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.290
High resolution limit [Å]2.2102.210
Number of reflections59867
Completeness [%]26.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP9.5298PEG 3000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

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