1Y43

crystal structure of aspergilloglutamic peptidase from Aspergillus niger

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1993-11-30
Detector	FUJI
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
格子定数 [Å]	55.100, 70.700, 38.300
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	10.000 - 1.400
R因子	0.197
Rwork	0.197
R-free	0.22600
Structure solution method	MIR
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	100.000	1.440
分解能 [Å] (高)	1.400	1.400
独立反射数	29424
完全性 [%]	97.5	82.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	2.1	298	ammonium sulfate, dimethyl sulfoxide, glycine, pH 2.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K