1XV8

Crystal Structure of Human Salivary Alpha-Amylase Dimer

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
格子定数 [Å]	150.812, 72.270, 91.106
格子定数 [度]	90.00, 102.80, 90.00

精密化法

残基	20.000 - 3.000
Rwork	0.228
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jxj
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.415
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000	3.110
分解能 [Å] (高)	3.000	3.000
Rmerge_l_obs		0.307
独立反射数	18579
完全性 [%]	96.3	95.5
冗長性	4.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.5		0.2 M Ca acetate, 0.1 M Na cacodylate (pH 6.5), 18% PEG 8K, VAPOR DIFFUSION, HANGING DROP, temperature 100K