1XI8
Molybdenum cofactor biosynthesis protein from Pyrococcus furiosus Pfu-1657500-001
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 63.547, 116.599, 147.226 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 42.954 - 2.504 |
| Rwork | 0.306 |
| R-free | 0.34300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fc5 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (refmac_5.2.0005) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.590 |
| 分解能 [Å] (高) | 2.500 | 5.380 | 2.500 |
| Rmerge_l_obs | 0.051 | 0.025 | 0.316 |
| Total number of observations | 4062 | 3050 | |
| 独立反射数 | 37143 | ||
| 完全性 [%] | 96.6 | 99.3 | 81.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 291 | 25.5%w/v PEG-8000, 0.17M ammonium sulfate, 15%v/v glycerol, pH no buffer, modified microbatch, temperature 291K |
