1XI6
Extragenic suppressor from Pyrococcus furiosus Pfu-1862794-001
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9700 |
| Spacegroup name | I 41 2 2 |
| 格子定数 [Å] | 81.613, 81.613, 293.871 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 26.860 - 2.800 |
| R因子 | 0.27224 |
| Rwork | 0.271 |
| R-free | 0.29828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dk4 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (refmac_5.2.0005) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.490 |
| 分解能 [Å] (高) | 2.400 | 5.170 | 2.400 |
| Rmerge_l_obs | 0.136 | 0.093 | 0.254 |
| Total number of observations | 2180 | 640 | |
| 独立反射数 | 16734 | ||
| 完全性 [%] | 83.4 | 99.5 | 33.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 6.2 | 291 | 0.3-0.9M ammonium dihydrogen phosphate, 30%v/v glycerol, 0.07M MES, pH 6.2, modified microbatch, temperature 291K |
| 1 | modified microbatch | 6.2 | 291 | 0.3-0.9M ammonium dihydrogen phosphate, 30%v/v glycerol, 0.07M MES, pH 6.2, modified microbatch, temperature 291K |
| 1 | modified microbatch | 6.2 | 291 | 0.3-0.9M ammonium dihydrogen phosphate, 30%v/v glycerol, 0.07M MES, pH 6.2, modified microbatch, temperature 291K |
